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زیست شناسی::
کدون توقف
A stop codon is required for translation to cease at the correct location.
A gene of interest can be fitted with var ious sequences that enhance translation and facilitate both secretion and purifica tion, such as a Kozak sequence (K), signal sequence (S), protein affinity tag (T), proteolytic cleavage site (P), and stop codon (SC).
An amber suppressor tRNA is a modified tRNA that can insert an amino acid into a protein in places where the mRNA contains an amber codon, UAG, which normally is a stop codon that directs the cessation of protein synthesis.
To prevent the translational fusion of proteins whose mRNAs normally contain a UAG stop codon with downstream proteins, in vivo suppression is always less than 100% and is often dependent upon the nucleotides surrounding the
The start codon is highlighted in green, and the stop codons are in red.
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